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Not like oligo(dT) primers, these primers usually are not template unique and they are ideal for DNA synthesis working with Klenow fragments with DNA templates or for cDNA synthesis employing reverse transcriptase with mRNA templates.
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Working with them instead of common primers without having modifying other factors of the load may lead to improved stress and potentially risky benefits. Constantly seek the advice of by far the most present reloading data When thinking about decision of primer. four. Exactly what are the safety precautions I should get when managing primers over the reloading approach?
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